Practical One –Clinical Chemistry
Clinical chemistry
Section A: Urine testing
Analysis of CA’s urine sample using ‘Labstix’
Now answer the following questions, being careful to think about what each test is designed to demonstrate and justifying the answer.
- Comment on each parameter from the ‘Labstix’ data above. Are there any abnormalities? (5 marks for each, 6 total)
| Parameter | Value (give units where appropriate) | High level in urine known as: | Abnormality |
| Ketone |
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| Protein |
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| Glucose |
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| Blood |
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Section B: Serum testing
- Which enzyme are you measuring in your serum sample? Explain the relationship with the red colour observed in some samples. (3 marks)
- Why do we include a STOP reaction in the experiment? (2 marks)
- How can we ensure standardisation of results between different clinical laboratories? (1 mark)
- Briefly describe the overall structure of Alkaline Phosphatases. (1 mark)
- What are necessary at each catalytic site for alkaline phosphatase enzymatic activity? (2 marks)
Alkaline phosphatase measurement
According to the method sheet, carry out the experiment on the serum sample from CA provided and use the readings provided in table below.
Reading
| Distilled water/zero | 0.000 |
| Control sample | 0.108 |
| Test Sample | 1.034 |
| Standard | 0.246 |
- Using the worked example as a guide (see the practical protocol), calculate the activity of alkaline phosphatase in CA’s serum sample from the readings provided in the table above. Show your working below. (5 marks)
- Input your IU/L value below. How does your value compare with the reference range provided in the practical session (also on the DLE)? (1 mark)
